LOCUS       40924_49027        3745 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD003, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3745)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3745)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..410
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    408..428
                     /note="GC; GC-adaptor for seamless assembly, flanking
                     antibiotic resistance markers or Escherichia coli origins 
                     to be assembled with a cyanobacterial replicon; synthetic"
     misc_feature    429..434
                     /label=NheI
                     /note="NheI"
     CDS             complement(515..1327)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1328..1425)
                     /note="PaphI; upstream region of aphI conserved between
                     pPMAQK1 and various plasmids,derived from INSD accession 
                     GU933126"
                     /regulatory_class="promoter"
     misc_feature    1434..1439
                     /label=AgeI
                     /note="AgeI"
     misc_feature    1440..1460
                     /note="C2G; GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers, functional devices or custom
                     sequences; synthetic"
     misc_feature    1458..1463
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    1482..1487
                     /label=XbaI
                     /note="XbaI"
     primer_bind     complement(1524..1540)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1548..1564)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1572..1602)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1617..1638)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1926..2514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2688..3545)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3546..3650)
                     /label=AmpR promoter
     misc_feature    3676..3681
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgaggatatc gcgcgcgcgc
      421 gcgcggatgc tagccaatgg cttattttcc tgctaattgg taataccatg gtaccgctct
      481 gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
      541 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
      601 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
      661 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
      721 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
      781 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
      841 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
      901 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
      961 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     1021 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     1081 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     1141 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     1201 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     1261 aatcagcatc catgttggaa tttaatcgcg gcctggagca agacgtttcc cgttgaatat
     1321 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     1381 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggaccggta
     1441 tcccgccgcc gccgccggat atcctcggta cccggggatc ctctagagtc gacctgcagg
     1501 catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1561 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1621 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1681 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     1741 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1801 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1861 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1921 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1981 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2041 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2101 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2161 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2221 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2281 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2341 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2401 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2461 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2521 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2581 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2641 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2701 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2761 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2821 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2881 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2941 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3001 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3061 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3121 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3181 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3241 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3301 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3361 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3421 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3481 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3541 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3601 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3661 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     3721 aggcgtatca cgaggccctt tcgtc
//
